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The Multichannel Segmentation architecture is designed to be used in new applications. It was initially tested for the 4 input series, 3 segment output MICCAI BraTS example case, and was successfully reapplied for segmentation of striatum and cerebellum in rat brain dopaminergic PET. The process of preparing the data to reapply the architecture, the training, and the evaluation of the outcome is described in this case study.